Automatic and Quantitative Measurement of Protein-Protein Colocalization in Live Cells
نویسندگان
چکیده
منابع مشابه
Automatic and quantitative measurement of protein-protein colocalization in live cells.
We introduce a novel statistical approach that quantifies, for the first time, the amount of colocalization of two fluorescent-labeled proteins in an image automatically, removing the bias of visual interpretation. This is done by estimating simultaneously the maximum threshold of intensity for each color below which pixels do not show any statistical correlation. The sensitivity of the method ...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2004
ISSN: 0006-3495
DOI: 10.1529/biophysj.103.038422